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Strep-A Exact Clearview

Catalogue Number: 
25 Tests
Throat Swab

A rapid test for the visual, qualitative detection of Group A Streptococcal antigen directly from throat swabs


Beta-hemolytic Group A Streptococcus is a major cause of upper respiratory infection such as tonsillitis, pharyngitis, and scarlet fever. Early diagnosis and treatment of Group A Streptococcal pharyngitis has been shown to reduce the severity of symptoms and further complications, such as rheumatic fever and glomeru- lonephritis. Conventional methods used for the detection of the disease depend on the isolation and subsequent identification of the organism. These methods often require 24-48 hours to complete. Developments of immunological techniques which can detect Group A Streptococcal antigen directly from throat swabs allow physicians to diagnose and administer therapy immediately.


Assay Time: 5 minutes

Sample Type: Throat swab


The Clearview Exact Strep A Cassette utilizes a two-site sandwich immunoassay technology for the detec- tion of Group A Streptococcal antigen. The test consists of a membrane strip that has been precoated with rabbit anti-Strep A antibody and a colored rabbit anti-Strep A polyclonal antibody-colloid gold conjugate pad that is placed at the end of the membrane. During testing, the Strep A antigen is extracted from the throat swab using Extraction Reagents 1 and 2. The extracted solution is then added to the cassette’s sample well. The Strep A antigen reacts with colored anti- body-colloidal gold conjugate to form Strep A antigen-antibody complexes. The mixture then moves chro- matographically across the membrane to the immobilized rabbit anti-Strep A antibody at the test line region. If Strep A antigen is present in the specimen, a colored sandwich of antibody / Strep A antigen / gold conju- gate antibody is formed on the test line. Absence of a colored line at the test line region indicates a nega- tive result. Regardless of the presence of Strep A antigen, as the extracted mixture continues to move laterally across the membrane to the control line region; a colored line at the control region will always appear. The presence of this colored line serves as verification that sufficient volume has been added and proper flow occurred.