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Malaria Rapid Test Paramax-3

Catalogue Number: 
50320025
Qty/Size: 
25 Tests
Sample: 
Whole blood, Capillary
Format: 
Cassette

Paramax-3 is a rapid, qualitative, two site sandwich immunoassay utilizing whole blood for the detection of P.falciparum specific histidine rich protein-2 (Pf. HRP-2), P.vivax specific pLDH and pan malaria specific pLDH.

Overview

Paramax-3 can be used for the specific detection of P. falciparum and P.vivax malaria, differentiation of other malarial species and for the follow up of antimalarial therapy. Four species of the Plasmodium parasites are responsible for malaria infections in human viz. P. falciparum, P.vivax, P.ovale and P.malariae. Of these, P. falciparum and P.vivax are the most prevalent. Early detection and differentiation of malaria is of utmost importance due to incidence of cerebral malaria and drug resistance associated with falciparum malaria and due to the morbidity associated with the other malarial forms. As the course of treatment is dependent on the species, differentiation between P. falciparum and P.vivax is of utmost importance for better patient management and speedy recovery.

Information

Assay Time: 20 minutes

Sample Type: Whole Blood, Capillary

 

Paramax-3 utilizes the principle of immunochromatography. As the test sample flows through the membrane assembly of the device after addition of the clearing buffer, the colored colloidal gold conjugates of anti-Pf. HRP-2 antibody, and anti pan specific pLDH antibody complexes the HRP-2 / corresponding pLDH in the lysed sample. This complex moves further on the membrane to the test region where it is immobilised by the monoclonal anti Pf. HRP-2 antibody and / or monoclonal anti P.vivax specific pLDH antibody and/or monoclonal pan specific pLDH antibody coated on the membrane leading to formation of a pink-purple colored band in the respective regions which confirms a positive test result. Absence of a colored band in the test region indicates a negative test result for the corresponding antigen. The unreacted conjugate along with the rabbit antisera colloidal gold conjugate and unbound complex if any,move further on the membrane and are subsequently immobilised by anti-rabbit antibodies coated on the membrane at the control region, forming a pink-purple band.This control band serves to validate the test performance.